Skip to main content
Skip to navigation

Shinghua Ding, PhD

Cramer W. LaPierre Professor
Biomedical, Biological and Chemical Engineering (BBCE)
Dalton Cardiovascular Research Center (DCRC)
Interdisciplinary Neuroscience Program (INP)

Office Location: 324E DCRC
Office Phone: 573-884-2489

Research Interests

Ischemic stroke, neurodegenerative diseases, glia-neuron interactions, reactive astrocytes, metabolism.

Research Description

The main research interest in Ding Lab is to investigate the molecular and cellular mechanisms in neurological diseases including ischemic stroke and neurodegenerative diseases. The lab uses preparations of primary cultured neural cells, human iPSCs, and mice as disease models and state-of-the art technologies including cell-type specific molecular genetics (e.g., transgenic mice, viral transduction, and CRISPR/Cas9 gene editing), in vivo two-photon imaging, electrophysiology, RNA-seq, metabolomics, human iPSCs-derived neuronal cells, behavioral tests, biochemical assays, immunocytochemistry, Western blot analysis, etc.

Professional Background

BS, Biochemical Engineering, Zhejiang University of Technology, China

PhD, Chemical and Biological Engineering, State University of New York at Buffalo

Postdoc training, Physiology/Biophysics, Thomas Jefferson University

Postdoc & Research Associate training, Neuroscience, University of Pennsylvania

Selected Publications

Samuel Lundt, Nannan Zhang, Luis Polo-Parada, Xinglong Wang, and Shinghua Ding. Dietary NMN supplementation enhances motor and NMJ function in ALS. Experimental Neurology 374: 114698, 2024.

Guangfu Wu, Nannan Zhang, Avi Matarasso, Ian Heck, Huijie Li, Wei Lu, Glenn Phaup, Michael Schneider, Yixin Wu, Zhengyan Weng, He Sun, Zan Gao, Xincheng Zhang, Stefan Sandberg, Dilruba Parvin, Elena Seaholm, Syed Kamrul Islam, Xueju Wang, Paul E. M. Phillips, Daniel Castro, Shinghua Ding, De-Pei Li, Michael R. Bruchas, Yi Zhang. Implantable aptamer-graphene microtransistors for real-time monitoring of neurochemical release in vivo. Nano Letters. 22: 3668−3677, 2022.

Guangfu Wu, Ian Heck, Nannan Zhang, Glenn Phaup, Xincheng Zhang, Yixin Wu, David E. Stalla, Zhengyan Weng, He Sun, Huijie Li, Zhe Zhang1, Shinghua Ding, De-Pei Li, and Yi Zhang. Wireless, battery-free push-pull microsystems for membrane-free neurochemical sampling in freely moving animals. Science Advances 8: eabn2277, 2022.

Victoria Cegielski, Rohan Chakrabarty, Shinghua Ding, Michael Wacker, Paula Monaghan-Nichols, and Xiang-Ping Chu. Acid-sensing ion channels in glial cells. Membranes 12: 119, 2022.

Nannan Zhang, Zhe Zhang, Ilker Ozden, Shinghua Ding. Imaging mitochondrial Ca2+ uptake in astrocytes and neurons in vitro and in vivo using cell-type specific mitochondria-targeted GCaMP5G/6s. J. Vis. Exp. DOI: 10.3791/62917-v, 2022.

Samuel Lundt, Shinghua Ding. NAD+ metabolism and diseases with motor dysfunction. Genes 12: 1776, 2021.

Zhe Zhang, Grace Sun, Shinghua Ding. Glial Cell Line-Derived Neurotrophic Factor and focal ischemic stroke. Neurochemical Research 46: 2638-2650, 2021.

Samuel Lundt, Nannan Zhang, Jun-Liszt Li, Zhe Zhang, Li Zhang, Xiaowan Wang, Ruisi Bao, Feng Cai, Wenzhi Sun, Woo-Ping Ge, Shinghua Ding. Metabolomic and transcriptional profiling reveals bioenergetic stress and activation of cell death and inflammatory pathways in vivo after neuronal deletion of NAMPT. Journal of Cerebral Blood Flow & Metabolism 41: 2116-2131, 2021.

Yadong Xu, Ganggang Zhao, Qihui Fei, Zanyu Chen, Zhe Zhang, Fufei An, Yangyang Chen, Yun Ling, Peijun Guo, Shinghua Ding, Guoliang Huang, Qing Cao, Zheng Yan. Pencil-paper on-skin electronics. PNAS 117: 18292-18301, 2020.

Nannan Zhang, Zhe Zhang, Rui He, Hailong Li, Shinghua Ding. GLAST-CreERT2 mediated deletion of GDNF increases brain damage and exacerbates long-term stroke outcomes after focal ischemic stroke in mouse model. Glia 68:2395-2414, 2020.

Samuel Lundt, Nannan Zhang, Xiaowan Wang, Luis Polo-Parada, Shinghua Ding. The effect of NAMPT deletion in projection neurons on the function and structure of neuromuscular junction (NMJ) in mice. Scientific Reports 10:99, 2020.

Yadong Xu, Bohan Sun, Yun Ling, Qihui Fei, Zanyu Chen, Xiaopeng Li, Peijun Guo, Nari Jeon, Shivam Goswami, Yixuan Liao, Shinghua Ding, Qingsong Yu, Jian Lin, Guoliang, Zheng Yan. Multiscale porous elastomer substrates for multifunctional on-skin electronics with passive-cooling capabilities. PNAS 117:205-213, 2020.

Xiaowan Wang, Zhe Zhang, Nannan Zhang, Hailong Li, Li Zhang, Christopher Baines, Shinghua Ding. Subcellular NAMPT-mediated NAD+ salvage pathways and their roles in bioenergetics and neuronal protection after ischemic injury. Journal of Neurochemistry 151: 732-748, 2019.

Nannan Zhang, Shinghua Ding. Imaging of mitochondrial and cytosolic Ca2+ signals in cultured astrocytes. Current Protocols in Neuroscience 82: 2.29.1–2.29.11, 2018.

Xiaowan Wang, Qiao Zhang, Ruisi Bao, Nannan Zhang, Luis Polo-Parada, Andrew Tarim, Aidan Alemifar, Xianlin Han, Heather M. Wilkins, Russell H. Swerdlow, Xinglong Wang, Shinghua Ding. Neuronal deletion of Nampt in adult mice leads to motor dysfunction, neurodegeneration, and death. Cell Reports 20: 2184-2200, 2017.

Xiaowan Wang, Shinghua Ding. Pre-B-cell colony-enhancing factor as a target for protecting against apoptotic neuronal death and mitochondrial damage in ischemia. Neural Regenerative Research 11: 1914-1915, 2016.

Xiaowan Wang, Hailong Li, Shinghua Ding. Pre-B-cell colony-enhancing factor protects against apoptotic neuronal death and mitochondrial damage in ischemia. Scientific Reports 6: 32416, 2016.

Gourav Roy Choudhury, Shinghua Ding. Reactive astrocytes and therapeutic potential in focal ischemic stroke. Neurobiology of Disease 85: 234-244, 2016.

Hailong Li, Yicheng Xie, Nannan Zhang, Yang Yu, Qiao Zhang, Shinghua Ding. Disruption of IP3R2-mdediated Ca2+ signaling pathway in astrocytes ameliorates neuronal death, brain damage while reducing behavioral deficits after focal ischemic stroke. Cell Calcium 58:565-576, 2015.

Hailong Li, Gourav Roy Choudhury, Nannan Zhang, Shinghua Ding. Photothrombosis induced focal ischemia as a model of spinal cord injury in mice. J. Vis. Exp. e53161, 2015.

Shinghua Ding. Astrocytic Ca2+ signaling and its role in modulating cerebral blood flow. Annals of Vascular Medicine and Research 1: 1006, 2014.

Shinghua Ding. Dynamic reactive astrocytes after focal ischemia. Neural Regenerative Research 9:2048-2052, 2014.

Xiaowan Wang, Hailong Li, Shinghua Ding. The effects of NAD+ on apoptotic neuronal death and mitochondrial biogenesis and function after glutamate excitotoxicity. In J Mol Sci 15:20449-20468, 2014.

Hailong Li, Xiaowan Wang, Nannan Zhang, Manoj K Gottipati, Vladimir Parpura, Shinghua Ding. Imaging of mitochondrial Ca2+ dynamics in astrocytes using cell-specific mitochondria-targeted GCaMP5G/6s: mitochondrial Ca2+ uptake and cytosolic Ca2+ availability via the endoplasmic reticulum store. Cell Calcium 56:457-466, 2014.

Hailong Li, Nannan Zhang, Hsinyun Lin, Yang Yu, Quan-Yu Cai, Lixin Ma, and Shinghua Ding. Histological, cellular and behavioral assessments of stroke outcomes after photothrombosis-induced ischemia in adult mice. BMC Neuroscience 15:58, 2014.

Shinghua Ding. In vivo astrocytic Ca2+ signaling in health and brain disorders (Review). Future Neurology 8:529-554, 2013.

Hailong Li, Nannan Zhang, Shinghua Ding. Inhibition of the group I mGluRs reduces acute brain damage and improves histological long-term outcomes after photothrombosis-induced ischemia. ASN NEURO 5(3): e00117, 2013.

Li Qin Zhang, Dilyara Cheranova, Margaret Gibson, Shinghua Ding, Daniel P. Heruth, Deyu Fang and Shui Qing Ye. RNA-seq reveals novel transcriptome of genes and their isoforms in human pulmonary microvascular endothelial cells treated with thrombin. PloS one 7:e31229, 2012.

Jing Bi, Hailong Li, Shui Qing Ye, Shinghua Ding. PBEF exerts a neuronal protection through its enzymatic activity and the reduction in mitochondrial dysfunction in in vitro ischemic models. Journal of Neurochemistry 120:334-346, 2012.

Yan He, Jiankun Cui, James C. Lee, Shinghua Ding, Malgorzata Chalimoniuk, Agnes Simonyi, Albert Y. Sun, Zezong Gu, Gary A. Weisman, W. Gibson Wood, and Grace Y. Sun. Prolonged exposure of neurons to oligomeric amyloid-beta causes impairment in NMDA receptor responses: protection by NADPH oxidase inhibitors and green tea (-)-epigallocatechin-3-gallate. ASN NEURO 3: e00050, 2011.

Yicheng Xie, Tiannan Wang, Grace Y. Sun, Shinghua Ding. Specific disruption of astrocytic Ca2+ signaling pathway in vivo by adeno-associated viral transduction. Neuroscience 170:992-10, 2010.

Weiping Zhang, Yicheng Xie, Tiannan Wang, Jing Bi, Hailong Li, Li Qin Zhang, Shui Qing Ye, Shinghua Ding. Neuronal protective role of PBEF in a mouse model of cerebral ischemia. Journal of Cerebral Blood Flow & Metabolism 30: 1962-1971, 2010.

Tiannan Wang, Wenju Cui, Yicheng Xie, Weiping Zhang, Shinghua Ding. Controlling the volume of the focal cerebral ischemic lesion through photothrombosis. The American Journal of Biomedical Sciences (AJBMS) 2:33-42, 2010.

Shinghua Ding, Tiannan Wang, Wenju Cui, Philip G. Haydon. Focal photo-thrombosis stimulates a sustained elevation of astrocytic Ca2+ signaling. Glia 57: 676-776, 2009.

Shinghua Ding, Tommaso Fellin, Yingzi Zhu, Yves Auberson, David Meaney, Douglas Coulter, Giorgio Carmignoto, Philip G. Haydon. Increased astrocytic Ca2+ oscillations stimulate neuronal excitotoxicity after status epilepticus. Journal of Neuroscience 27: 10674-84, 2007.

Conrad Messan, Shinghua Ding, Philip G. Haydon. Functional differentiation of human brain progenitor cells. Neuron Glia Biology 2:187-198, 2007.

Shinghua Ding, Conrad Messan, Peiying Li, Micheal E. Selzer, Marc A. Dichter, Philip G. Haydon. Murine brain progenitor cells have ability to differentiate into functional neurons and integrate into the CNS. Cell Transplantation 15:699-710, 2006.

Shinghua Ding, Lindsey Ingleby, Chris Ahern, Richard Horn. Investigating the putative glycine hinge in Shaker potassium channel. Journal of General Physiology 126: 213-226, 2005.

Shinghua Ding, Richard Horn. Effect of S6 tail mutations on charge movement in Shaker K+ channels. Biophysical Journal 84: 295-305, 2003.

Shinghua Ding, Frederick Sachs. Evidence for non-independent gating of P2X2 receptors expressed in Xenopus oocytes. BMC Neuroscience 3:17, 2002.

Shinghua Ding, Richard Horn. Tail end of the S6 segment: roles in permeation in Shaker K+ channels. Journal of General Physiology 120: 87-97, 2002.

Shinghua Ding, Richard Horn. Slow kinetics of immobilizing the voltage sensors in ion channels. Biochemistry 40(35):10707-10716, 2001.

Richard Horn, Shinghua Ding, and Hermann Gruber. Immobilization of moving parts of voltage-gated ion channels. Journal of General Physiology 116: 461-476, 2000.

Shinghua Ding, Frederick Sachs. Inactivation of P2X2 receptors by divalent cations. Journal of Physiology 222:199-214, 2000.

Shinghua Ding, Frederick Sachs. Ion permeation and block of P2X2 purinoceptors: single channel recordings. Journal of Membrane Biology 172: 215-223, 1999.

Shinghua Ding, Frederick Sachs. Single channel properties of P2X2 purinoceptors. Journal of General Physiology 113: 695-720, 1999.

Published by Dalton Cardiovascular Research Center, 1500 Research Park Drive, Columbia, MO 65211
Phone: 573-882-7588 Email: