Luis Polo-Parada, PhDAssociate Professor, Department of Medical Pharmacology and Physiology
Office Location: 302 DCRC
Office Phone: 573-884-4599
The heart is the first organ to form during embryogenesis, and its function is critical for the proper development and survival of the embryo. Although some information on ion-transport genes and their protein products in normal and diseased myocardial tissue is available, little is known about the role of cardiac extracellular matrix (ECM) proteins during cardiac development or in healthy and diseased adult hearts. Polo-Parada's interest is to elucidate the role of the ECM in the ionic-transport proteins and molecular basis of cardiac regional electrical specialization during development and in the adult heart. Nanotechnology is fast becoming one of the major areas of research in medicine and life science in general. Its appeal lies in the long dreamt of ability to investigate and manipulate matter at the level of individual atoms and molecules. Nanomedicine is the medical use of molecular-sized particles to deliver drugs, heat, light or other substances to specific cells in the human body. Engineering particles to be used in this way allows detection and/or treatment of diseases or injuries within the targeted cells, thereby minimizing the damage to healthy cells in the body. My main interest is in the development of nano drug delivery system, nanomaterials for imaging and diagnosis, bio/nano-sensor and nanomaterials for cell/tissue and organ manipulation.
The heart is the first organ to form during embryogenesis, and its function is critical for the proper development and survival of the embryo. Although some information on ion-transport genes and their protein products in normal and diseased myocardial tissue is available, little is known about the role of cardiac extracellular matrix (ECM) proteins during cardiac development or in healthy and diseased adult hearts. Polo-Parada's interest is to elucidate the role of the ECM in the ionic-transport proteins and molecular basis of cardiac regional electrical specialization during development and in the adult heart.
Nanotechnology is fast becoming one of the major areas of research in medicine and life science in general. Its appeal lies in the long dreamt of ability to investigate and manipulate matter at the level of individual atoms and molecules. Nanomedicine is the medical use of molecular-sized particles to deliver drugs, heat, light or other substances to specific cells in the human body. Engineering particles to be used in this way allows detection and/or treatment of diseases or injuries within the targeted cells, thereby minimizing the damage to healthy cells in the body. My main interest is in the development of nano drug delivery system, nanomaterials for imaging and diagnosis, bio/nano-sensor and nanomaterials for cell/tissue and organ manipulation.
- BS in physics and Mathematics, National Polytechnic Institute, Mexico.
- MS in physiology and neurobiology, University of Connecticut.
- PhD in neurosciences, Case Western Reserve University.
- Received a National Scientist Development Award from the American Heart Association.
- Member of the Society for Neuroscience, Biophysical Society and American Heart Association.
Sudhir Ravula, Jeremy B. Essner, Wendy A. L, Luis Polo-Parada, Roli Kargupta, Garret J. Hull, Shramik Sengupta, and Gary A. Baker. (2014). Sunlight-assisted route to antimicrobial plasmonic aminoclay catalysts. Nanoscale. In Press. DOI: 10.1039/C4NR04544K.
Francisco I. Ramirez-Perez, Gerardo Gutiérrez-Juárez, Sangho Bok, Keshab Gangopadhyay, Shubhra Gangopadhyay, Gary A. Baker and Luis Polo-Parada (2014). Dye-doped Organosilicate Nanoparticles as cell-preserving labels for photoacoustic signal generations. Journal of Biomedical Nanotechnology. In Press.
Jorge A. Castorena-Gonzalez, Marius C. Staiculescu, Christopher A. Foote, Luis Polo-Parada and Luis A. Martinez-Lemus (2014). The obligatory role of the actin cytoskeleton on inward remodeling induced by dithiothreitol activation of endogenous transglutaminase in isolated arterioles. Am J Physiol Heart Circ Physiolg. In Press.
Luis Polo-Parada, Gabriel Mettlack, Lauren Peca, Clinton T. Rubin, Florian Plattner and James A Bibb (2013). Enhancement of neuromuscula dynamics and strenght behavior using extremely low magnitude mechanical signals in mice. Journal of Biomechanics. In Press.Luis Polo-Parada
and Amol A. Modgi (2013) .Differences in Expression and Function in the Atrium versus Ventricle of the Sodium-Calcium Exchanger in the Embryonic Chicken Heart," ISRN Physiology, vol. 2013, Article ID 921527, 12 pages, 2013. doi:10.1155/2013/921527.
G. Gutiérrez-Juárez, H. A. Vela-Lira, M. Yánez-Limón, F. J. García-Rodríguez and Luis Polo-Parada (2013). Quantitative photoacoustic spectroscopy in the frequency domain. International Journal of Thermophysics. In Press.
Bryant C. Harris, Venumadhav Korampally, Craig Weilbaecher, Luis Polo-Parada, Sheila Grant, Shubhra Gangopadhyay. (2013). Protease biosensing on novel high surface area organosilicate nanoporous films. Sensors and Actuators B: Chemical. Volume 176, January 2013, Pages 351–359.
J.E. Alba-Rosales, G Ramos-Ortiz, M. Rodriguez, G Gutierrez-Juarez and L Polo-Parada (2013). In situ Characterization of Laser Ablation by Pulsed Photoacoustic: The Case of Organic Nanocrystal Synthesis. International Journal of Thermophysics. 1394-2, DOI 10.1007/s10765-013-1394-2.
Bok, S. Korampally, V, Dar CM, Folk Wr, Gangopadhyay K, Gangopadhyay S, and Polo-Parada L (2012). Femtogram-level Detection of Clostridium botulinum Neurotoxin Type A by Sandwich Immunoassay Using Nanoporous Substrate and Ultra-bright Fluorescent Suprananoparticles. Biosensor and Bioelectronics. Volume 41, 15 March 2013, Pages 409–416.
Korampally M, Apperson SJ, Staley CS, Castorena JA, Thirunvengadathan R, Gangopadhyay K, Mohan RR, Gosh A, Gangopandhyay S, Polo-Parada L. (2012) Transient pressure-mediated intranuclear delivery of FITC-Dextran into chicken cardiomyocytes by MEMS-based nanothermite reaction actuator. Sensors & Actuators: B. Chemical. Volumes 171–172, August–September 2012, Pages 1292–1296.
Tan J, Lee B, Polo-Parada L, Sengupta S. Kinetically Limited Differential Centrifugation in an inexpensive and readily-available alternative to Centrifugal Elutriation (2012). Biotechniques. Aug;53(2):104-8. doi: 10.2144/0000113853.
Bok S, Korampally V, Polo-Parada L, Mamidi V, Baker G, Gangopadhyay K, Folk WR. Dasgupta PK, Gangopadhyay S (2012). Confeito-like Assembly of Organosilicate1caged Fluorophores: Ultrabright Suprananoparticles for Fluorescence Imaging. Nanotechnology. 23, 175601 doi:10.1088/0957-4484/23/17/175601
Pérez-Solano R, Gutiérrez Juárez G,* Polo-Parada L* (2012). Modelo esféricamente simétrico de la señal fotoacústica en el dominio temporal producida por objetos micrométricos: el caso de células de melanoma in vitro. Superficies y Vacio. 25(2).
Pérez-Solano, R, Ramirez-Perez FI, Castorena Gonzalez JA, Anell EA; Gutiérrez-Juárez G,* Polo-Parada, L* (2012). An Experimental and Theoretical approach to the study of the photoacoustic signal produced by cancer cells. AIP Advances. 2, 011102 (2012); http://dx.doi.org/10.1063/1.3697852
Behringer EJ, Socha, MJ, Polo-Parada L, Segal, SS. (2011). Electrical conduction along endothelial cell tubes from mouse feed arteries: Confounding actions of glycyrrhetinic acid derivative. British Journal of Pharmacology. DOI: 10.1111/j.1476-5381.2011.01814.x.
Darr CM, Castorena J, Korampally V, Gangopadhyay S* Polo-Parada L*. (2011). A Comparative Evaluation of Microarray Slides as Substrates for the Development of Protease Assay Biosensors. Experimental and Molecular Pathology. 91(3):714-7
Polo-Parada L,* Maeno-Hikichi Y,* Kastanenka K, Landmesser LT. (2011). Frequency dependent modes of synaptic vesicle endocytosis and exocytosis at adult mouse neuromuscular junctions. The Journal of Neuroscience. 31(3):1093-1105.
Rutland CS, Polo-Parada L, Ehler E, Alibhai A, Thorpe A, Suren S, Emes RD, Patel B, Loughna S., Knockdown of embryonic myosin heavy chain reveals an essential role in the morphology and function of the developing heart., Development. 2011 Sep;138(18):3955-66.PMID: 21862559
Bagher P, Polo-Parada L, Segal SS., Microiontophoresis and micromanipulation for intravital fluorescence imaging of the microcirculation., J Vis Exp. 2011 Jun 10;(52). pii: 2900. doi: 10.3791/2900.PMID: 21694691
Maeno-Hikichi Y, Polo-Parada L, Kastanenka KV, Landmesser LT.,Frequency-dependent modes of synaptic vesicle endocytosis and exocytosis at adult mouse neuromuscular junctions.,J Neurosci. 2011 Jan 19;31(3):1093-105.PMID: 21248134
Gutiérrez-Juárez G, Gupta SK, Weight RM, Polo-Parada L, Papagiorgio C, Bunch JD, Viator JA., Optical Photoacoustic Detection of Circulating Melanoma Cells In Vitro., Int J Thermophys. 2010 May 1;31(4):784-792.PMID: 20730036
Gutierrez-Juarez G, Gupta SK, Al-Shaer M, Polo-Parada L, Dale PS, Papageorgio C, Viator JA.,Detection of melanoma cells in vitro using an optical detector of photoacoustic waves., Lasers Surg Med. 2010 Mar;42(3):274-81.PMID: 20333746
Wang S, Polo-Parada L, Landmesser LT., Characterization of rhythmic Ca2+ transients in early embryonic chick motoneurons: Ca2+ sources and effects of altered activation of transmitter receptors., J Neurosci. 2009 Dec 2;29(48):15232-44.PMID: 19955376
Polo-Parada, L,Zhang X, and Modgi A. (2009). Cardiac Cushions Modualte Action Potential Phenotype During Heart Development. Developmental Dynamics. In Press.
Sen A, Barizuddin S, Hossain, M, Polo-Parada L, Gillis KD, Gangopadhyay S. (2009). Prefeential cell atachment to nitrogen-doped diamon-like carbon (DLC:N) for the measurement of quantal exocytosis. Biomaterials. In Press.
Hata K, Polo-Parada L, Landmesser LT (2007) . Selective targeting of different cell adhesion molecule isoforms during motoneuron myotube synapse formation in culture and the switch from an immature to mature form of synaptic vesicle cycling. J Neurosci; 27(52):14481-14493
Chen Y, Sharp AH, Hata K, Yunker AM, Polo-Parada L, Landmesser LT, McEnery MW (2007). Site-directed antibodies to low-voltage-activated calcium channel CaV3.3 (alpha1I) subunit also target neural cell adhesion molecule-180. Neuroscience 145(3):981-996.
Polo-Parada L,Chan S-A, Smith C. (2006). An activity-dependent increased role for L-type calcium channels in exocytosis is regulated by adrenergic signaling in chromaffin cells. Neuroscience 43(2):445-459.
Javsek M. Jaworski,A., Polo-Parada, L, Kim N. Fan J., Landmesser, LT., Burden SJ. (2006) . CD24 is expressed by myofiber synaptic nuclei and regulates synaptic transmission. Proc. Natl. Acad Sci. USA, 103(16);6374-6379.
Polo-Parada, L. Plattner F. Bose, C. Landmesser LT. (2005) . NCAM acting via a conserved C-terminal domain and requiring MLCK activity is essential for effective transmission with repetitive stimulation. Neuron 46(6);917-931.
Chan, S-A., Polo-Parada, L., Landmesser, L. T. and Smith, C. (2005) . Adrenal Chromaffin cells exhibit altered granule size and recruitment competence in KCAM knockout mice. J Neurophysiol 94(2);1037-1047.
Chan, S-A, Polo-Parada, L., Smith C. (2005) . Action potential stimulation reveals and increased role for P/Q-calcium channel-dependent exocytosis in mouse adrenal tissue slices. Arch Biochem Biophys, 435(1);65-73.
Polo-Parada, L. Bose, C. Plattner, F. and Landmesser L. T. (2004) . Distinct roles of different NCAM isoforms in synaptic maturation reveled by analysis of NCAM 180kDa isoform deficient mice. J Neurosci; 24(8);1852-1864.
Polo-Parada, L. Bose, C. and Landmesser L. T. (2001) . Alterations in Transmission, Vesicle Dynamics, and Transmitter Release Machinery at NCAM-Deficient Neuromuscular Junctions. Neuron 32(5); 815-828.
Rafuse, V. F., Polo-Parada, L. and Landmesser, L. T. (2000) . Structural and functional alterations of neuromuscular junctions in CNAM deficient mouse. J. Neurosci. 20(17); 6529-6539.
Polo-Parada L. and Pilar, G. (1999). k and m opioid reverse the somatostatin inhibition of Ca2+ currents and dorsal root ganglion neurons. J. Neurosci. 19(13):5213-5227.
Gray, D.B., Polo-Parada, L., Pilar, G., Eang, P., Metzger, R. R., Klann, E. and Meriney, S. D. (1999) . A nitric oxide/cGMP-dependent protein kinase pathway alters transmitter release and inhibition by somatostatin at a site downstream of calcium entry. J. Neurochem. 72(5):1981-90.
Alanis, J., Arguello, C and Polo L. (1997) . Effects of heparin on the electrophysiological and mechanical properties of chick embryo hearts. J. Cell and Mol Card.29(9):2503-11.
Polo-Parada, L. and Korn, S.J. (1997) . Block of N-type calcium channels in chick sensory neurons by external sodium. J. Gen. Phy. 109(6):693-702.