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Shinghua Ding, PhD

Associate Professor, Biological Engineering
Office Location: 324E Dalton Cardiovascular Research Center
Office Phone: 573-884-2489

Research Interests

Cerebral ischemia

Research Description

Cerebral ischemia (ischemic stroke) is a leading neural disorder that causes brain damage and human death, and has a major impact on public health. Though various mechanisms by which ischemia induce brain damage have been proposed, clinically there is limited therapeutic approach that is effective to brain recovery after ischemia. Therefore, my research generally focuses on seeking and identifying new mechanisms that can reduce brain injury and improving long-term outcomes after stroke. My research focuses on two distinct but related areas: 1) Glial function and role in stroke; 2) Neuronal mechanisms in brain protection in stroke. We use mice (in vivo) and primary cultured cells (in vitro) including neurons and astrocytes isolated from mouse brains as experimental preparations. We use both in vivo and in vitro ischemic models for ischemic study. Approaches including molecular biology, fluorescent imaging including 2-in vivo two-photon (2-P) microscopy, confocal and epi-fluorescent microscopy, biochemistry, electrophysiology, cell culture, and immunocytochemistry are integrated in our research.

Professional Background

2013-Peer review study section member for BRAIN 5, American Heart Association (AHA).

2013-2016: Chair of the Membership Committee for American Society of Neurochemistry (ASN).

2011-2013: Member of the Membership Committee for American Society of Neurochemistry (ASN).

Session chair of colloquium titled “Glial dysfunction and role in brain ischemia”, 43rd Annual meeting of American Society for Neurochemistry, March 3-7, 2012, Baltimore.

Invited speaker for symposium titled ‘Mitochondrial Ca2+ signaling in life and death of glial cells’. Presentation title: “Interplay between mitochondrial and cytosolic Ca2+ signaling in astrocytes during ischemia”. XI European Meeting on Glial Cell Function in Health and Disease. Berlin Germany, July 3-6, 2013.

Invited speaker and organizer for Cardiovascular Day. Presentation title: “PBEF plays a critical role in brain protection after ischemia”. Cardiovascular Day, University of Missouri-Columbia, February 21, 2012.

Grant reviewer for Research Board Grant, University of Missouri System. November, 2011.

Grant reviewer for Medical Research Council (MRC), UK. December, 2010.

Invited speaker for Cardiovascular Day. Presentation title: “Astrocytic responses to focal cerebral ischemia”. Cardiovascular Day, University of Missouri-Columbia, February 26, 2007.

Invited seminar speaker from multiple universities including A.T. Still University, University of Missouri-Columbia, University of Missouri-Kansas City, Missouri University of Science and Technology, Shanghai Institute of Neuroscience (China), Zhejiang University (China), Wuhan University (China), Merck & Co., et al.

Invited reviewer of multiple scientific journals including Journal of General Physiology, Neuroscience, ASN NEURO, British Journal of Pharmacology, NeuroImage, Molecular Pharmacology, Journal of Vascular Research, PLoS Ones, etc.

PhD, Chemical and Biological Engineering, State University of New York at Buffalo, Buffalo, New York.

MS, Chemical and Biological Engineering, State University of New York at Buffalo, Buffalo, New York.

MS, Biochemical Engineering, Dalian Polytechnic University.

BS, Biochemical Engineering, Zhejiang University of Technology, Hangzhou, China.

2007-present: assistant rofessor, Biological Engineering/Dalton Cardiovascular Research Center, University of Missouri, Columbia, Mo.

2005-2006: research associate, Department of Neuroscience, University of Pennsylvania, Philadelphia.

2002-2005: Postdoctoral fellow, Department of Neuroscience, University of Pennsylvania, Philadelphia.

1999-2002: Postdoctoral fellow, Department of Molecular Physiology, Thomas Jefferson University.

1991-1993: instructor, Department of Chemical Engineering & Biotechnology, Zhejiang University.

1989-1991: assistant instructor, Department of Chemical Engineering & Biotechnology, Zhejiang University.

Selected Publications

Hailong Li, Nannan Zhang, Grace Sun, Shinghua Ding*. Inhibition of the group I mGluRs reduces acute brain damage and improves long-term histological outcomes after photothrombosis-induced ischemia. ASN NEURO 5(3), e00117, 2013. PMID:23772679.

Li Qin Zhang, Dilyara Cheranova, Margaret Gibson, Shinghua Ding, Daniel P. Heruth, Deyu Fang4 and Shui Qing Ye1,2. RNA-seq reveals novel transcriptome of genes and their isoforms in human pulmonary microvascular endothelial cells treated with thrombin. PloS ONE 7:e31229, 2012.

Jing Bi3, Hailong Li2, Shui Qing Ye, Shinghua Ding*. PBEF exerts a neuronal protection through its enzymatic activity and the reduction in mitochondrial dysfunction in in vitro ischemic models. Journal of Neurochemistry 120:334-346, 2012.  

Shinghua Ding. In vivo imaging of Ca2+ signal in astrocytes using two-photon laser scanning fluorescent microscopy. Methods in Molecular Biology 814:545-554, 2012.

Zhang W, Xie Y, Wang T, Bi J, Li H, Zhang LQ, Ye SQ, Ding S., Neuronal protective role of PBEF in a mouse model of cerebral ischemia., J Cereb Blood Flow Metab. 2010 Dec;30(12):1962-71. Epub 2010 May 19.PMID: 20485294

Ding S, Wang T, Cui W, Haydon PG., Photothrombosis ischemia stimulates a sustained astrocytic Ca2+ signaling in vivo., Glia. 2009 May;57(7):767-76.PMID: 18985731

Shinghua Ding*, Tommaso Fellin*, Yingzi Zhu*, Yves Auberson, David Meaney, Douglas Coulter, Giorgio Carmignoto, Philip G. Haydon. Increased astrocytic Ca2+ oscillations stimulate neuronal excitotoxicity after status epilepticus (*- equal contribution), accepted by Journal of Neuroscience.

Conrad Messan1, Shinghua Ding1,2, Philip G. Haydon. Functional Differentiation of Human Brain Progenitor Cells (1-equal contribution, 2-corresponding author). Neuron Glia Biology 2:187-198, 2007.

Shinghua Ding1, Conrad Messan1, Peiying Li, Micheal E. Selzer, Marc A. Dichter, Philip G. Haydon. Murine Brain Progenitor Cells Have Ability to Differentiate into Neurons (1-equal contribution, 2-corresponding author). Cell Transplantation 15:699-710, 2006.

Shinghua Ding, Lindsey Ingleby, Chris Ahern, Richard Horn. Investigating the putative glycine hinge in Shaker potassium channel. Journal of General Physiology 126: 213-226, 2005.

Shinghua Ding, Richard Horn. Effect of S6 Tail Mutations on Charge Movement in Shaker K+ Channels. Biophysical Journal 84: 295-305, 2003.

Shinghua Ding, Frederick Sachs. Evidence for Non-Independent Gating of P2X2 Receptors Expressed in Xenopus oocytes. BMC Neuroscience 3:17, 2002.

Shinghua Ding, Richard Horn. Tail End of the S6 Segment: Roles in Permeation in Shaker K+ Channels. Journal of General Physiology 120: 87-97, 2002.

Shinghua Ding, Richard Horn. Slow Kinetics of Immobilizing the Voltage Sensors in Ion Channels. Biochemistry 40(35):10707-10716, 2001.

Richard Horn, Shinghua Ding, and Hermann Gruber. Immobilization of Moving Parts of Voltage-Gated Ion Channels. Journal of General Physiology 116: 461-476, 2000.

Shinghua Ding, Frederick Sachs. Inactivation of P2X2 Receptors by Divalent Cations. Journal of Physiology 222:199-214, 2000.

Shinghua Ding, Frederick Sachs. Ion Permeation and Block of P2X2 Purinoceptors: Single Channel Recordings. Journal of Membrane Biology 172: 215-223, 1999.

Shinghua Ding, Frederick Sachs. Single Channel Properties of P2X2 Purinoceptors. Journal of General Physiology 113: 695-720, 1999.

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